STUDIES ON RENAL GLUTAMINASE I

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Glutaminase from Pig Renal Cortex

A method for preparing highly purified glutaminase (EC 3.5.1.2, L-glutamine amidohydrolase) from pig renal cortex is described. The main steps consist of sodium sulfate fractionation followed by alternative solubilization by dialysis against Tris-HCI buffer and precipitation with phosphateborate. The linal enzyme preparation contains no impurities which could be detected by sucrose gradient cen...

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Renal Glutaminase Activities in Vitamin

Glutamine has been implicated in the functioning of the central nervous system (l), in muscle metabolism (a), in transamination (3), in detoxification (4), and as a precursor of urinary ammonia (5). An excellent review of these and other possible r&es of glutamine in metabolism has been presented by Archibald (6). It would seem that glutamine acts as a mobile reserve of ammonia and glutamic aci...

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Partial purification and properties of renal glutaminase.

In 1935, Krebs (1) suggested that there may be several glutaminases. A useful preparation of renal ‘Lglutaminase” was described by Archibald (2) ; Greenstein and Carter (3) demonstrated the existence of two types of glutaminases which were activated by a-keto acids and phosphate, respectively. The mechanism of the a-keto acid-activated glutaminases has been demonstrated by Meister and Tice (4),...

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Renal glutaminase activities in vitamin B6-deficient rats.

Glutamine has been implicated in the functioning of the central nervous system (l), in muscle metabolism (a), in transamination (3), in detoxification (4), and as a precursor of urinary ammonia (5). An excellent review of these and other possible r&es of glutamine in metabolism has been presented by Archibald (6). It would seem that glutamine acts as a mobile reserve of ammonia and glutamic aci...

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Renal glutaminase adaptation and ammonia excretion in infant rats.

The purpose of this investigation was to determine the role of enzyme adaptation in the response of ammonia excretion to acidosis in developing rats. The response of renal ammonia excretion was low in infant rats (7-12 days old) following administration of a single dose of acidifying salt (5 mmol NH4CL/kg). However, repeated administration (2 times daily) of the salt increased ammonia excretion...

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ژورنال

عنوان ژورنال: The Japanese Journal of Urology

سال: 1966

ISSN: 0021-5287,1884-7110

DOI: 10.5980/jpnjurol1928.57.4_323